The game begins in Peach's Castle. Professor Elvin Gadd is demonstrating a new invention--the Nintendo DS Horror. He is accompanied by Mario, Luigi, Peach, Daisy, Yoshi, Birdo, and Toad. He switches it on and suddenly, a vortex comes out of the top screen and sucks in everything in the Mushroom Universe.
Where Are We?
Mario and Luigi wake up in a grassy meadow. They both stand up and ponder where they are. Suddenly, Toad runs up to them. "Mario! Luigi! The others are over there!" Toad runs off to the left of the screen and then offscreen. Luigi follows him, leaving Mario alone to catch up with them.