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Luigi-acts as a super guide and completes the level for the player.
Yoshi-eats enemies, then uses the eggs for offense.
Fire Mario/Fire Luigi-an upgrade to Mario/Luigi, can throw fireballs that burn enemies.
Ice Mario/Ice Luigi-an upgrade to Mario/Luigi, can throw ice balls that freeze enemies, making them easier to attack.
Bob-omb Buddy-shoots cannon balls at the enemies.
Peach-gives Mario more health.
Daisy-gives Luigi more health (Super Guide only).
Donkey Kong-attacks are powerful, but have short range.
Diddy Kong-throws bananas at a long range. These bananas make enemies slip and enemies are temporarily inactive.
POW Block-defeats all enemies on-screen.