Professor E. Gadd
Professor E. Gadd will appear on the stage with the Poltergust 5000, and will proceed to either burn the opponents with fire, or freeze them with ice. He then disappears.
Chunky Kong appears on the stage and preforms his Primate Punch, and finishes the opponent with a blast from his Pineapple Launcher. He will then disappear afterwards.
Jimmy T. appears on the stage and proceeds turn it into a disco parlor, and dances. The effects on the fighters is similar to Luigi's Negative Zone attack from Brawl.
Muddy Mole just runs around dropping bombs as he does. The bombs will explode shortly after, they don't do that much damage but still send opponents flying a bit.
When summoned, the Nintendog will pop up and block the screen for a few seconds before something offscreen grabs his attention.
Bio Rex & Octoman
When summoned Bio Rex and Octoman will attempt to ram into opponents with the Deep Claw and the Big Fang. However they crash into each other and their machines will explode on the opponents. After being launched out of the machines, the duo disappears.
When summoned, Dai Goroh will run around the stage slashing opponents with his katana in the same way that his father Samurai Goroh did in previous Smash titles.
Kat & Ana