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The player's aim is to defeat The Dark Kingdom as Peach, Nana, Samus, Daisy or Zelda or try to defeat Bowser and his henchmen as Mario, Luigi, Wario, Yoshi or as one of other 6 characters. The game features 26 characters (23 from the story, 3 appears in the game).
There are 15 playable characters (25 if you count the bosses) in the game. If the player chooses Peach, Nana, Samus, Daisy or Zelda, the player will fight The Dark Kingdom starting with Waluigite. The other 10 characters will fight against King Dedede, King K. Rool, Gannondorf, Kamek and Bowser.
- Princess Peach Toadstool (as Moon-Peach)
- Nana (as Merc-Nana) and Popo
- Samus Aran (as Mars-Samus)
- Princess Daisy (as Jup-Daisy)
- Princess Zelda (as Venus-Zelda)
- Donkey Kong
- Diddy Kong
- Koji and Nagisa
- Fred Boneburger
There are 10 bosses the player must face. If you are using Peach, Nana, Samus, Daisy or Zelda, you must confront the Dark Kingdom starting with Waluigite. The other 10 characters will fight the other 5 bosses whil you'll always start with King Dedede. They are also playable, one of the Dark Kingdom members will fight 5 non-Dark Kingdom bosses and the vice-versa.
The Dark Kingdom
These are the bosses that one of the Sailor Soliders must face in order
These are the bosses that the non-Sailor Soliders must face
- King Dedede
- King K. Rool
The Final Boss of the game. She is an AI only boss character and player must her in 2 forms. Once beaten, you'll get to see a character's ending.