Yoshi - A poor dinosaur from Yoshi's Island, who has been given the burden of helping get Baby Mario and Baby Luigi back home. His abilities include swallowing enemies whole, turning them into eggs, and hurling the eggs into other enemies.
Samus - A nine year old girl, raised by the chozo on Zebes. Her unique athletic abilities are the result of an infusion of chozo blood. She is armed with a paralyzer and combat training.
Olimar - An alien man from a far away planet called Hocotate. When Sass, Link and Ike's ship collides into Olimar's, both ships crash land on Distant Planet. Olimar can fight with hand to hand combat, but usually lets the Pikmin to do his bidding.
Prince Mush - Mush, the champion of the Glitz Pit, in Glitzville. He has discovered some dark secrets about the Glitz Pit, and has been receiving anonymous threats. He fights using his agility and martial arts.
Bly - A creation of the Ancients. Not much is known about him.
Beldam - A shadow bent on finding the Crystal Stars, to open the Thousand Year Door. She has an alliance with the Heartless and Doopliss.
Doopliss - A Duplighost who has discovered a way to gain powerful magic. The only way to gain this magic is by erasing everyone who knows his name. To accomplish this, he has allied with Beldam.