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- Princess Peach
- Princess Daisy
- Bowser Jr.
- Shy Guy
- Fly Guy
- Goomba = Get by completing leaf cup on 150cc in 1st place
- Paragoomba = Get by completing mushroom cup singles in 1st,
- Wario = Have 5 different MVPs on baseball
- Waluigi = Defeat Chief Chilly in racing
- Snifit = Defeat Bowser in Volleyball challenge
- Fly Snifit = Complete 100cc or 150cc star cup
- Baby Mario = Complete 1-2 in Super Mario Tennis
- Baby Luigi = Play multiplayer 7 times
- Baby Peach = Unlock every character
- Baby Daisy = Get 10,000 coins