Mario Bros. or Mario Brothers may refer to:
- Mario and Luigi, the Mario Brothers.
- Baby Mario and Baby Luigi, who are also sometimes referred to as the "Baby Mario Bros."
- Multiple uses of "Mario Bros." in video games released by Nintendo.
- The original Mario Bros. game.
- The Mario & Luigi series.
- Multiple pages on Fantendo:
- Mario Bros. 2010, a game by UnijuXP (t∣b∣c)
- Mario Bros. (2011), a game by Silver the Hedgehogfan (t∣b∣c)
- Mario Bros. Ultra, a game by Awesome Rowlet and Epic Popplio (t∣b∣c)
- Mario Bros. Adventure!, a game by Ultra Kuzon (t∣b∣c)
- Mario Bros. Returns, a game by MushShroom (t∣b∣c)
- Mario Brothers, a game by Rewire387 (t∣b∣c)