|Living Town||Mushroom Kingdom|
|First Appearance||Mario Mysteries|
|Latest Appearance||Super Mario Sunshine IV: Paint Peril|
Very little is known about Janie's past. SILPH Project created her as a clone of Daisy to do their will- however, she had Daisy's memories and morals, so Janie thought that she was Daisy. As such, she thought SIPLH had simply captured her. She escaped and encountered the real Daisy two days later, who realized that Janie was a clone. She told Janie this, trying to help her, but this made Twice's mind crack. In her fury at being tricked, Janie attacked Daisy. The ensuing battle led to the clone being permanently disfigured and the real Daisy in the emergency room. The two have been rivals ever since.
Janie Twice is generally portrayed sympathetically. Her mind is split halfway good, half evil. She has more than once wished to return to a normal life.
In this Purpleverse Day 2010 game, Janie appears as one of the main villains. She was summoned by Sunnyscythe with Scythe Guy and later CrackedEgg to kill Purple Guy and YoshiEgg Nook, as well as complete other tasks leading to the destruction of the world. During the game, she is very sadistic and twisted, as always. All of the villains develop a sort of crush on her as the game progresses, especially Sunnyscythe, who makes romantic remarks about her every so often and defends her in danger. However, it is unknown if these feelings are actual or meerly a joke.
Janie Twice appears yet again in the Black Diamond game Dark Purple. As of now, it is unknown what her role is, but it is guessed that she will be a major villain.
Janie Twice is in this upcoming game, she will be a major vilain in this game having one of Prof E Gadd's magic inventions. She kidnaps Daisy and attempts to kill her. It is unknown what she does after this.