Mario, Pauline, and a few Mini Marios are taking a walk. Mario and Pauline plan to have a picnic with a few of Mario's friends. While they were walking to the park, Donkey Kong watched them with binoculars on top of a building. It made Donkey Kong very livid and thought they excluded him in a special event. Donkey Kong hopped off the building and landed in front of Mario, Pauline, and the Mini Marios. DK crushed a Mini Mario while the other 3 screamed "Mama Mia!" and dashed behind Mario as the ape grabbed Pauline and ran away with her. Mario chased after DK while the Mini Marios were depressed and watching the crushed Mini.
After DK fell down into tires, Mario grabbed Pauline's hand and jumped off the platform and the whole tower exploded. Mario and Pauline landed safely on air tires. DK jumped out of the tires and punched Mario, causing Mario to fall back. DK grabbed Pauline again as he dashed to a building and jump on top of it and ran away with Pauline. Mario chased after DK on the sidewalk since he can't jump that high.
DK was almost defeated and just as Mario was thinking to finish him off, dozens of Mini Marios came to attack DK. One Mini Mario came up to DK and touched him lightly, causing DK to fall down. Mario came up to DK and put out his hand, and DK grabbed Mario's hand to get up. "Want to come to the park with us?" Mario asked. Donkey Kong nodded as Mario, DK, Pauline, and the Mini Marios jumped down the building. After 5 minutes, every one invited to Mario's picnic came and started the picnic.
|Mario||Everyone's favorite plumber returns to the basics! Mario has to stop Donkey Kong and save his friend Pauline!|
|White Mario||A playable character only in Multiplayer Mode.|
|Blue Mario||A playable character only in Multiplayer Mode.|
|Green Mario||A playable character only in Multiplayer Mode.|
|Donkey Kong||The main antagonist of this sequel. DK has captured Pauline again but with a few tricks up his sleeve.|
|Pauline||Pauline has been captured by DK again! It's up to Mario to save her.|
|Mini Mario||Mini Marios make cameo appearances in the background of the levels and in cutscenes.|
|Luigi||Makes a cameo at Mario's picnic.|
|Princess Peach||Makes a cameo at Mario's picnic|
|Toad||Makes a cameo at Mario's picnic.|