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Mario is walking with Luigi on the side of the road. Then, the Koopa Man, Krusher, runs by with a stone Peach. Luigi follows him, not knowing he'd be stone in a second. Mario chases. He takes out an item. He eats it and becomes big. Mario is on his way. He stops Krusher, who has delivered Luigi and Peach to Bowser. Mario must traverse the 7 worlds to stop Bowser...or an even bigger evil.
World 1: Mushroom Kingdom
- BOSS: Bowser
Afterward, the Pi-antas come and take the statues and flee to Isle Delfino, now deserted.
World 2: Deserted Delfino
- BOSS: Pi-anta Gangsters
After the Pi-anta Gangsters are taken down, they flee to their ship. Mario gives chase.
World 3: Rusher Sea
- BOSS: Sea Dragon
The Sea Dragon is taken down. The Rusher Sea is calm. And...a cruise ship approaches.
World 4: Pi-anta Cruiser
- BOSS: Pi-anta Gangsters Improved
The return of the Pi-anta gangsters shocks Mario. Literally. Now, they are all equipped with stun guns. They are taken down, but Mario is thrown overboard.
Mini-World: Rusher Sea (A Mini-World is a world with one level.)
World 5: Dark Fields
- BOSS: Agony Tiger
The Agony Tiger is made of pure darkness. Mario extinguishes it, but finds something behind it; the Luigi statue. Mario cannot revive it, so he takes it to the Underworld.
World 6: Underworld
- BOSS: Pi-anta King
The Pi-anta King in the Underworld? That means... Something else awaits at Pi-anta Towers...
World 7: Pi-anta Towers
- BOSS: Pi-anta Army
The Pi-antas are almost all taken out when the Pi-anta Gangsters ask for the un-revived Luigi statue. The final boss awaits at the portal.
Mini-World: 3.14159365 Dimension
The Pi-anta King is in his throne here. He has the Luigi statue, and the Peach statue, and they are awakening... They turn into Luigi and Peach, but something is wrong. They turn big and all...strange. Final Boss time.
FINAL BOSS: Pi Luigi and Pi Peach
The bosses are beaten, the Pi-antas dead, the world back to normal, and Luigi and Peach alive.