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Boss Battles returns from Cartoon All-Star Melee, with the only changes being the mix of bosses. The mode is unlocked after the player completes The King of Darkness for the very first time. All bosses are as follows...
|Judge Doom||Who Framed Roger Rabbit?|
|The Mad Doctor|
|The Dirty Bubble|
|Sir Miles Axelrod|
|Judge Calude Frollo||The Hunchback of Notredame|
|The King of Darkness|