Barney Bubble appears in Super Mario World 2: Yoshi's Island. When Yoshi was traversing the Monkey's Favorite Lake, he came across two of these creatures on a bridge. Yoshi jumped on the pink creature, and it spat bubbles out of its mouth. Yoshi could grab these bubbles with his tongue and spit them out at other enemies. Eventually, Yoshi ignored the two Barney Bubbles and went on his merry way.
- His name is a pun on the cartoon character Barney Rubble.
- In the Game Boy Advance remake of Yoshi's Island, two more Barney Bubbles appear in the Endless World of Yoshis, one of the six new levels in the remake.